How We Can Support the Drug Development Pipeline

DNA Methylation profiling and Epigenetics

DNA methylation is thought to play a critical role in the regulation of gene expression, guiding normal cellular development and maintaining tissue identities. Alterations in DNA methylation, or epigenetic changes because they do not alter the genetic code, affect the structure of DNA and are known to be associated with carcinogenesis due to transcription inactivation.

Methylation Patterns In Cancer

 Patterns of methylation seen in cancer are very different to that of normal tissue and are usually associated with localised hypermethylation of CpG islands. This causes genetic instability and damage leading to tumour formation.

Methylation profiling can be a useful tool in understanding the underlying molecular basis of disease and can provide important insight into drug targets. Quantitative methylation measurement at the single-CpG-site level offers the highest resolution for understanding epigenetic changes.

Methylation Profiling Using Illumina

Using Illumina, molecular scientists at Source BioScience can provide methylation profiling with single-CpG resolution. For the investigation of genome-wide methylation signatures, using the HumanMethylation27 BeadChip our researchers can quantitatively interrogate 27,578 CpG loci covering more than 14,000 genes at single-nucleotide resolution. This high-density panel lets researchers profile up to 12 samples in parallel.

GoldenGate

Using 96-sample array matrix, GoldenGate Methylation Panels we can enable the interrogation of up to 1,536 loci per sample and provide our pharma/biotech researchers with the high-throughput solution for their methylation research. We can also offer Cancer Panels that target 1,505 CpG loci selected from 807 cancer-related genes. Researchers can also design Custom GoldenGate Methylation Panels that target specific CpG sites of interest providing flexibility as well as a robust assay. For more details, please contact us.

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